R&D Assay Development Intern
- MADISON, WI, United States
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Job details
- Posting language
- English
- Posted
Original job description
JOB REQUIREMENTS: OUR TEAM: Our group builds bioluminescent and fluorescent tools that let researchers see what happens inside living cells and in biochemical systems. As an intern, you will contribute to projects that may include: -Bioluminescent reporter and complementation technologies: NanoLuc luciferase-based systems such as NanoBiT and HiBiT for measuring protein abundance, protein-protein interactions, and protein degradation. -Immunoassay-based signaling detection: homogeneous, no-wash bioluminescent formats for measuring intracellular proteins and post-translational modifications such as phosphorylation. -Fluorescence-based methods: fluorescent labeling (for example HaloTag ligands), fluorescence imaging, and plate-reader fluorescence, used alongside luminescence for orthogonal validation. -Enzyme activity assays: biochemical and cell-based detection of kinases and other signaling enzymes. -Drug discovery applications: assay miniaturization, plate-based and high-throughput screening formats, and compound profiling. YOUR ROLE: -Perform cell culture, transfections, and cell-based assays in multi-well formats. -Run biochemical assays, including reagent preparation, buffer optimization, and titrations. -Operate luminometers, fluorescence plate readers, and imaging instruments. -Collect, organize, and analyze data (dose-response curves, signal-to-background, Z\' factor, assay window.) -Document experiments clearly in an electronic lab notebook. -Follow protocols with direction, and learn to troubleshoot and ask the right questions when results are unexpected. -Present results and contribute ideas at group meetings. CORE DUTIES: 1. Apply Promega proprietary technologies to develop intracellular target detection and signaling assays. 2. Become competent in the application of one or more techniques. 3. Work with cultured cells to develop detection assays. 4. Understand role in team environment. 5. Interact effectively with co-workers and team members. 6. Follow project plan with assistance. 7. Work at bench. 8. Demonstrates inclusion through their own words and actions and is accountable for a safe workspace. Acts with kindness, curiosity and respect for others. 9. Embracing and being open to incorporating Promega\'s 6 Emotional & Social Intelligence (ESI) core principles in daily work. 10. Understands and complies with ethical, legal and regulatory requirements applicable to our business. (This should be the last numbered statement under the Core Duties) KEY QUALIFICATIONS: 1. Must be currently enrolled in a degree program in the life sciences (biology, chemistry, etc.). 2.... For full info follow application link. Diversity is important at Promega. We are proud to be an Equal Opportunity Employer, and make employment decisions without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran status, disability, or any other protected class. ***** APPLICATION INSTRUCTIONS: Apply Online: ipc.us/t/A6D375B93CAB459F Qualified females, minorities, and special disabled veterans and other veterans are encouraged to apply.