Protein Purification Scientist

Required Skills & Experience

• Hands-on industry experience in protein purification, particularly recombinant protein purification.
• Demonstrated experience purifying proteins from E. coli-based expression systems.
• Experience with insect and/or mammalian cell-based protein purification.
• Understanding of common protein purification and chromatography techniques.
• Experience with size-exclusion chromatography and routine protein quality assessment.
• Hands-on aseptic cell culture experience.
• Master’s degree or higher preferred,

Job Description

We are seeking a hands-on Protein Purification Scientist to support a Project within one of our large pharmaceutical scientific organizations. This individual will focus primarily on protein purification while also supporting protein expression and cell culture activities. The ideal candidate will have experience purifying recombinant proteins produced in E. coli, insect, and mammalian cell systems and be comfortable performing routine protein characterization and quality-control activities.

Key Responsibilities

• Perform protein purification from E. coli, insect cell, and mammalian cell expression systems.
• Execute routine protein purification techniques using chromatography columns and protein purification equipment.
• Perform size-exclusion chromatography (SEC) and other routine purification/quality-control activities to evaluate protein purity.
• Run protein gels and conduct routine biochemical characterization to assess purified protein quality and purity.
• Support protein expression and cell culture activities in addition to downstream purification.
• Perform mammalian-cell transfection using plasmids to support recombinant protein expression.
• Support viral infection-based expression workflows involving insect or other applicable cell systems.
• Maintain strong aseptic cell culture/subculture techniques when working with cell-based protein expression systems.