Postdoctoral Associate

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.Be You.

The Duke University Department of Obstetrics and Gynecology is seeking an on-site Postdoctoral Associate.

Molecular biology experience and extensive cell culture experience required. Specific experience and aptitude in stem-cell based trophoblast and endometrial organoid and co-culture models and their perturbations (human and macaque). Bioinformatic analyses are required. Supervision of other trainees required. Physical requirements: walking, carrying, sitting, stooping, reaching, crouching, hearing/listening, standing, grasping, lifting 15 lbs.

4-5 years of research experience or an equivalent time period of post-baccalaureate training

Minimum Requirements:

• Ph.D. or equivalent doctorate (e.g. Sc.D., M.D., D.V.M.).
• Should be used for post-doctoral fellows if the candidate has completed all of the requirements for a degree, but the degree has not been formally conferred; the candidate may present evidence of completion of the degree requirements, together with a statement documenting the date on which the degree is to be conferred.

Be Bold.

Work Performed

• 2D cell culture and 3D stem cell culture, human peripheral blood mononuclear cell (PBMC) isolation and culture, decidual immune cell isolation and culture
• Prepare cell culture samples for immunofluorescence staining (IF) or immunohistochemistry staining (IHC) including embedding samples for paraffin sections, staining fixed samples with antibodies and imaging using light, fluorescence and confocal microscopes available in the Duke University LMCF. Analyze images using image analysis software, obtain data for publication.
• Prepare protein for western blots and ELISA
• Design and perform molecular assays including but not limited to DNA extraction, RNA extraction, PCR, qPCR and prepare single cell and single nuclei for single cell and single nuclei RNA sequencing testing. Possible performance of ChIP seq and ATAC seq. Bioinformatic analyses of these data will be required
• Use standard and CRISPR knock-down and knock-out methods in stem cell derived culture systems
• Prepare, present and discuss data at lab meetings and journal clubs
• Assist in figure preparation for manuscripts and presentations
• Assist in manuscript writing, preparation for publication and manuscript revision
• Maintain a scientific record of any experimental plans and processes performed
• Maintain laboratory and associated equipment in a clean, well-ordered, and functional manner. Keep supplies on hand with a well-planned inventory and ordering system.
• Train and support undergraduate and graduate students and clinical fellows in their laboratory work

Choose Duke.

Join our award-winning team as identified by Forbes magazine as America’s Best Large Employer 2024 and be part of an inclusive culture that values excellence, innovation, and discovery. As an organization, we have exciting opportunities to be forward-thinking leaders in our field. We want talented individuals to join us, examine our current operations, and create innovative solutions that will revolutionize and enhance the way we approach our work.

Duke University is consistently ranked among the top universities worldwide and is renowned for its cutting-edge research across disciplines.

Beyond the engaging work, you'll also benefit from Duke's competitive benefits package including health insurance plans, generous paid time off, retirement programs with employer contributions, tuition assistance for employees and their children, and more.

Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas - an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.